Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses.
Identifieur interne : 002805 ( Main/Exploration ); précédent : 002804; suivant : 002806Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses.
Auteurs : Nidhi Pareek [Suède] ; Thomas Gillgren ; Leif J. JönssonSource :
- Bioresource technology [ 1873-2976 ] ; 2013.
Descripteurs français
- KwdFr :
- Adsorption (MeSH), Cellulase (métabolisme), Chromatographie sur gel (MeSH), Cinétique (MeSH), Endo-1,4-beta xylanases (métabolisme), Facteurs temps (MeSH), Fagus (composition chimique), Hydrolyse (MeSH), Lignine (métabolisme), Masse moléculaire (MeSH), Picea (composition chimique), Polyosides (métabolisme), Populus (composition chimique), Porosité (MeSH), Protéines (métabolisme), Spectroscopie infrarouge à transformée de Fourier (MeSH), Température (MeSH), bêta-Glucosidase (métabolisme).
- MESH :
- composition chimique : Fagus, Picea, Populus.
- métabolisme : Cellulase, Endo-1,4-beta xylanases, Lignine, Polyosides, Protéines, bêta-Glucosidase.
- Adsorption, Chromatographie sur gel, Cinétique, Facteurs temps, Hydrolyse, Masse moléculaire, Porosité, Spectroscopie infrarouge à transformée de Fourier, Température.
English descriptors
- KwdEn :
- Adsorption (MeSH), Cellulase (metabolism), Chromatography, Gel (MeSH), Endo-1,4-beta Xylanases (metabolism), Fagus (chemistry), Hydrolysis (MeSH), Kinetics (MeSH), Lignin (metabolism), Molecular Weight (MeSH), Picea (chemistry), Polysaccharides (metabolism), Populus (chemistry), Porosity (MeSH), Proteins (metabolism), Spectroscopy, Fourier Transform Infrared (MeSH), Temperature (MeSH), Time Factors (MeSH), beta-Glucosidase (metabolism).
- MESH :
- chemical , metabolism : Cellulase, Endo-1,4-beta Xylanases, Lignin, Polysaccharides, Proteins, beta-Glucosidase.
- chemistry : Fagus, Picea, Populus.
- Adsorption, Chromatography, Gel, Hydrolysis, Kinetics, Molecular Weight, Porosity, Spectroscopy, Fourier Transform Infrared, Temperature, Time Factors.
Abstract
Protein adsorption onto eight lignocellulosic substances (six lignin preparations and two hemicelluloses) was investigated at pH 4.8 and at two different temperatures (4°C and 45°C). The kinetics of the adsorption of cellulase, xylanase, and β-glucosidase were determined by enzyme activity measurements. The maximum adsorption capacities, the affinity constants and the binding strengths varied widely and were typically higher for the lignins than for the carbohydrates. As indicated by BET and gel permeation chromatography, different substances had widely different surface area, pore size, weight average molecular weight, and polydispersity index, but these properties were difficult to relate to protein binding. In most cases, an increase in temperature from 4°C to 45°C and a low content of carboxylic acid groups, as indicated by Fourier-Transform Infra-Red (FTIR) spectroscopy, resulted in increased protein adsorption capacity, which suggests that hydrophobic interactions play an important role.
DOI: 10.1016/j.biortech.2013.08.121
PubMed: 24045193
Affiliations:
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Jönsson</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:24045193</idno><idno type="pmid">24045193</idno><idno type="doi">10.1016/j.biortech.2013.08.121</idno><idno type="wicri:Area/Main/Corpus">002461</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">002461</idno><idno type="wicri:Area/Main/Curation">002461</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">002461</idno><idno type="wicri:Area/Main/Exploration">002461</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses.</title><author><name sortKey="Pareek, Nidhi" sort="Pareek, Nidhi" uniqKey="Pareek N" first="Nidhi" last="Pareek">Nidhi Pareek</name><affiliation wicri:level="1"><nlm:affiliation>Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden.</nlm:affiliation><country xml:lang="fr">Suède</country><wicri:regionArea>Department of Chemistry, Umeå University, SE-901 87 Umeå</wicri:regionArea><wicri:noRegion>SE-901 87 Umeå</wicri:noRegion></affiliation></author><author><name sortKey="Gillgren, Thomas" sort="Gillgren, Thomas" uniqKey="Gillgren T" first="Thomas" last="Gillgren">Thomas Gillgren</name></author><author><name sortKey="Jonsson, Leif J" sort="Jonsson, Leif J" uniqKey="Jonsson L" first="Leif J" last="Jönsson">Leif J. Jönsson</name></author></analytic><series><title level="j">Bioresource technology</title><idno type="eISSN">1873-2976</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adsorption (MeSH)</term><term>Cellulase (metabolism)</term><term>Chromatography, Gel (MeSH)</term><term>Endo-1,4-beta Xylanases (metabolism)</term><term>Fagus (chemistry)</term><term>Hydrolysis (MeSH)</term><term>Kinetics (MeSH)</term><term>Lignin (metabolism)</term><term>Molecular Weight (MeSH)</term><term>Picea (chemistry)</term><term>Polysaccharides (metabolism)</term><term>Populus (chemistry)</term><term>Porosity (MeSH)</term><term>Proteins (metabolism)</term><term>Spectroscopy, Fourier Transform Infrared (MeSH)</term><term>Temperature (MeSH)</term><term>Time Factors (MeSH)</term><term>beta-Glucosidase (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adsorption (MeSH)</term><term>Cellulase (métabolisme)</term><term>Chromatographie sur gel (MeSH)</term><term>Cinétique (MeSH)</term><term>Endo-1,4-beta xylanases (métabolisme)</term><term>Facteurs temps (MeSH)</term><term>Fagus (composition chimique)</term><term>Hydrolyse (MeSH)</term><term>Lignine (métabolisme)</term><term>Masse moléculaire (MeSH)</term><term>Picea (composition chimique)</term><term>Polyosides (métabolisme)</term><term>Populus (composition chimique)</term><term>Porosité (MeSH)</term><term>Protéines (métabolisme)</term><term>Spectroscopie infrarouge à transformée de Fourier (MeSH)</term><term>Température (MeSH)</term><term>bêta-Glucosidase (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Cellulase</term><term>Endo-1,4-beta Xylanases</term><term>Lignin</term><term>Polysaccharides</term><term>Proteins</term><term>beta-Glucosidase</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Fagus</term><term>Picea</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Fagus</term><term>Picea</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Cellulase</term><term>Endo-1,4-beta xylanases</term><term>Lignine</term><term>Polyosides</term><term>Protéines</term><term>bêta-Glucosidase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adsorption</term><term>Chromatography, Gel</term><term>Hydrolysis</term><term>Kinetics</term><term>Molecular Weight</term><term>Porosity</term><term>Spectroscopy, Fourier Transform Infrared</term><term>Temperature</term><term>Time Factors</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adsorption</term><term>Chromatographie sur gel</term><term>Cinétique</term><term>Facteurs temps</term><term>Hydrolyse</term><term>Masse moléculaire</term><term>Porosité</term><term>Spectroscopie infrarouge à transformée de Fourier</term><term>Température</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Protein adsorption onto eight lignocellulosic substances (six lignin preparations and two hemicelluloses) was investigated at pH 4.8 and at two different temperatures (4°C and 45°C). The kinetics of the adsorption of cellulase, xylanase, and β-glucosidase were determined by enzyme activity measurements. The maximum adsorption capacities, the affinity constants and the binding strengths varied widely and were typically higher for the lignins than for the carbohydrates. As indicated by BET and gel permeation chromatography, different substances had widely different surface area, pore size, weight average molecular weight, and polydispersity index, but these properties were difficult to relate to protein binding. In most cases, an increase in temperature from 4°C to 45°C and a low content of carboxylic acid groups, as indicated by Fourier-Transform Infra-Red (FTIR) spectroscopy, resulted in increased protein adsorption capacity, which suggests that hydrophobic interactions play an important role. </div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">24045193</PMID><DateCompleted><Year>2014</Year><Month>08</Month><Day>29</Day></DateCompleted><DateRevised><Year>2013</Year><Month>10</Month><Day>14</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1873-2976</ISSN><JournalIssue CitedMedium="Internet"><Volume>148</Volume><PubDate><Year>2013</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Bioresource technology</Title><ISOAbbreviation>Bioresour Technol</ISOAbbreviation></Journal><ArticleTitle>Adsorption of proteins involved in hydrolysis of lignocellulose on lignins and hemicelluloses.</ArticleTitle><Pagination><MedlinePgn>70-7</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.biortech.2013.08.121</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0960-8524(13)01372-2</ELocationID><Abstract><AbstractText>Protein adsorption onto eight lignocellulosic substances (six lignin preparations and two hemicelluloses) was investigated at pH 4.8 and at two different temperatures (4°C and 45°C). The kinetics of the adsorption of cellulase, xylanase, and β-glucosidase were determined by enzyme activity measurements. The maximum adsorption capacities, the affinity constants and the binding strengths varied widely and were typically higher for the lignins than for the carbohydrates. As indicated by BET and gel permeation chromatography, different substances had widely different surface area, pore size, weight average molecular weight, and polydispersity index, but these properties were difficult to relate to protein binding. In most cases, an increase in temperature from 4°C to 45°C and a low content of carboxylic acid groups, as indicated by Fourier-Transform Infra-Red (FTIR) spectroscopy, resulted in increased protein adsorption capacity, which suggests that hydrophobic interactions play an important role. </AbstractText><CopyrightInformation>Copyright © 2013 The Authors. Published by Elsevier Ltd.. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Pareek</LastName><ForeName>Nidhi</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Department of Chemistry, Umeå University, SE-901 87 Umeå, Sweden.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gillgren</LastName><ForeName>Thomas</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Jönsson</LastName><ForeName>Leif J</ForeName><Initials>LJ</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>08</Month><Day>29</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Bioresour Technol</MedlineTA><NlmUniqueID>9889523</NlmUniqueID><ISSNLinking>0960-8524</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011134">Polysaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011506">Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>11132-73-3</RegistryNumber><NameOfSubstance UI="C036909">lignocellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>8024-50-8</RegistryNumber><NameOfSubstance UI="C007916">hemicellulose</NameOfSubstance></Chemical><Chemical><RegistryNumber>9005-53-2</RegistryNumber><NameOfSubstance UI="D008031">Lignin</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.21</RegistryNumber><NameOfSubstance UI="D001617">beta-Glucosidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.4</RegistryNumber><NameOfSubstance UI="D002480">Cellulase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.8</RegistryNumber><NameOfSubstance UI="D043364">Endo-1,4-beta Xylanases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000327" MajorTopicYN="N">Adsorption</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002480" MajorTopicYN="N">Cellulase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002850" MajorTopicYN="N">Chromatography, Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D043364" MajorTopicYN="N">Endo-1,4-beta Xylanases</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D029964" MajorTopicYN="N">Fagus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006868" MajorTopicYN="N">Hydrolysis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008031" MajorTopicYN="N">Lignin</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008970" MajorTopicYN="N">Molecular Weight</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D028222" MajorTopicYN="N">Picea</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011134" MajorTopicYN="N">Polysaccharides</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016062" MajorTopicYN="N">Porosity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011506" MajorTopicYN="N">Proteins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017550" MajorTopicYN="N">Spectroscopy, Fourier Transform Infrared</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001617" MajorTopicYN="N">beta-Glucosidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Cellulase</Keyword><Keyword MajorTopicYN="N">Lignin</Keyword><Keyword MajorTopicYN="N">Mannan</Keyword><Keyword MajorTopicYN="N">Protein adsorption</Keyword><Keyword MajorTopicYN="N">Xylan</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>08</Month><Day>19</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>08</Month><Day>20</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>9</Month><Day>19</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>9</Month><Day>21</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>8</Month><Day>30</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">24045193</ArticleId><ArticleId IdType="pii">S0960-8524(13)01372-2</ArticleId><ArticleId IdType="doi">10.1016/j.biortech.2013.08.121</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Suède</li></country></list><tree><noCountry><name sortKey="Gillgren, Thomas" sort="Gillgren, Thomas" uniqKey="Gillgren T" first="Thomas" last="Gillgren">Thomas Gillgren</name><name sortKey="Jonsson, Leif J" sort="Jonsson, Leif J" uniqKey="Jonsson L" first="Leif J" last="Jönsson">Leif J. Jönsson</name></noCountry><country name="Suède"><noRegion><name sortKey="Pareek, Nidhi" sort="Pareek, Nidhi" uniqKey="Pareek N" first="Nidhi" last="Pareek">Nidhi Pareek</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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